亮點

?11.01%脂肪水平顯著促進了凡納濱對蝦卵巢發育階段IV和V的百分比。

?11.01%脂肪水平顯著提高了血淋巴和卵巢中ECR和RXR的基因表達。

?10.14%-14.65%的脂肪水平顯著上調卵巢發育相關蛋白的表達（Vtg和Hsd3b1）。 

摘要

本研究旨在評估飼料中脂肪水平對凡納濱對蝦卵巢發育的影響。實驗設置五個脂肪水平：8.87%、10.14%、11.01%、12.88%和14.65%，實驗為期14周。結果表明，11.01%脂肪組卵巢中n-3長鏈多不飽和脂肪酸含量在所有處理中最高。11.01%脂肪組蝦的卵巢發育至IV期和V期的比例最高。該組血淋巴中的甘油三酯、總膽固醇、雌二醇、5-羥色胺、卵黃蛋白原以及卵巢中卵黃蛋白原含量均顯著高于其他處理組。11.01%脂肪飼料顯著上調了卵巢發育相關基因(pcna、sesn、vg1、vgr和vtg)的表達，而大于10.14%脂肪組則顯著提高了卵巢發育相關蛋白(Vtg/α-Tubulin和Hsd3b1/α-Tubulin)的表達水平。11.01%脂肪組對蝦的腦部和卵巢中蛻皮相關基因(sad、rxr、e75、hr3和hr4)的表達量最高。飼料脂肪水平顯著影響脂代謝相關基因的表達。綜上所述，11.01%脂肪飼料有助于肝胰腺中外源性卵黃蛋白原的合成，并通過卵黃蛋白原受體將其轉運至卵巢，進而促進卵巢內源性卵黃蛋白原的生成。

Highlights

?Dietary 11.01?% lipid significantly promoted percentage of IV and V ovarian developmental stages of L. vannamei.

?Dietary 11.01?% lipid significantly enhanced concentration of ECR and RXR in hemolymph and ovary.

?The protein expression of Vtg and Hsd3b1 significantly up-regulated by dietary 10.14?%?~?14.65?% lipid.

Abstract

The purpose of this research was to evaluate the effect of dietary lipid levels on ovarian development for Pacific white shrimp(Litopenaeus vannamei). Five levels of lipid were prepared to contain 8.87?%, 10.14?%, 11.01?%, 12.88?% and 14.65?%, respectively. The feeding expreiment lasted for fourteen weeks. The results evidenced that 11.01?% lipid diet group showed the maximum n-3 LC-PUFA in ovary among all treatments. The highest proportion of ovarian maturation to stage IV and stage V occurred at the treatment with 11.01?% lipid. The treatment with 11.01?% lipid showed the highest TG, TCHO, E2, 5-HT, VTG in hemolymph, and VTG in ovary among all treatments. Dietary 11.01?% lipid notably up-regulated the expression of genes relevant to ovary development such as pcna, sesn, vg1, vgr and vtg in ovary, dietary excessive 10.14?% lipid can notably up-regulated the expression of protein related to ovary development such as Vtg / α-Tubulin and Hsd3b1 / α-Tubulin. The highest expression of brain and ovarian genes related to molting such as sad, rxr, e75, hr3 and hr4 existed at the treatment with 11.01?% lipid. Dietary lipid significantly affected lipid metabolism-related gene expression. In conclusion, dietary 11.01?% lipid contributed to the synthesis of hepatopancreatic exogenous vitellinogen, and transportation to the ovary via the vitellogenin receptor, further promoting the production of ovarian endogenous vitellinogen.

翻譯:伏曉曉

原文：Lei T, Li M, Wang J, et al. Dietary lipid regulates ovarian development, molting and lipid metabolism via the RXR/ECR pathway in female Litopenaeus vannamei[J]. Aquaculture, 2025: 743086.

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